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Protein

Alamelu Nachiyappan
in Biochemistry and Molecular Biology

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Hi,

 

How long the protein samples( cell Lysate) can be stored in 4 degree? If its there at 4 degree for more than 12 hours won't the Proteins be degraded?Can it be still used for the experiments like WB?

 

 

Alamelu

 

 

 

If it is a raw lysate without any inhibitors and nothing that prevents proteolysis you will have significant degradation. It is usually best to store it e.g. after precipitation or freeze drying, if possible.

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If it is a raw lysate without any inhibitors and nothing that prevents proteolysis you will have significant degradation. It is usually best to store it e.g. after precipitation or freeze drying, if possible.

 

thank you

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