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Chelex 100

semeli
in Biochemistry and Molecular Biology

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hi all!

 

i want to use chelex 100 sodium form (Sigma 50-100 dry mesh) in order to remove calcium traces from my protein but i dont know how to use it.

 

any suggestion is always wellcome.

 

cheers,

s

Check Dunn, M.F., et al., Biochemistry, vol. 19, 718 (1980). There are variation with differences in speed and recovery, but if you do not really intend to build a column or something similar the described approach should work fine. Just remember that the binding is highly pH dependent.

Edited by CharonY

  • 1 month later...

Normally w/v % is used. I.e. you just weigh in the desired amount of Chelex (say 5g) and then add ultrapure water (in this case 100 ml) to create a suspension with the given percentage.

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