Verbatim_25 Posted May 27, 2012 Share Posted May 27, 2012 Hi People, Is it possible to label the N terminal of a peptide with Rhodamine B. As u know Rhodamine B has a carboxylic acid and under coupling conditions, that is HBTU/HOBt and DIPEA in DMF, could be easily incorporated. This is what I guess...but I wanted to ask whether u have any experience in the incorporation of this fluorophore to any peptide sequence. I didn't find any protocol in which is reported...and therefore I feel a little bit afraid about if it is possible to attach it... Thanks, Verbatim:25 Link to comment Share on other sites More sharing options...
CharonY Posted May 30, 2012 Share Posted May 30, 2012 There are certainly rhodamine esters or isothicyanates that can be used for labeling of primary amines. Check Invitrogen (now probably part of Thermo), for instance. Link to comment Share on other sites More sharing options...
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