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Rhodamine B to label the N terminal peptide

Verbatim_25
in Biochemistry and Molecular Biology

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Hi People,

 

Is it possible to label the N terminal of a peptide with Rhodamine B. As u know Rhodamine B has a carboxylic acid and under coupling conditions, that is HBTU/HOBt and DIPEA in DMF, could be easily incorporated. This is what I guess...but I wanted to ask whether u have any experience in the incorporation of this fluorophore to any peptide sequence. I didn't find any protocol in which is reported...and therefore I feel a little bit afraid about if it is possible to attach it...

 

Thanks,

 

Verbatim:25

There are certainly rhodamine esters or isothicyanates that can be used for labeling of primary amines. Check Invitrogen (now probably part of Thermo), for instance.

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