afsaneh Posted May 14, 2012 Share Posted May 14, 2012 Hi DH10B is host of my plasmid , but we dont have DH10B in lab. can I use top10 or DH5a instead of DH10B? (base of my plasmid is pbluescript KS+ ) Link to comment Share on other sites More sharing options...
CharonY Posted May 15, 2012 Share Posted May 15, 2012 IIRC it is a pBR322 derivative with nothing terribly fancy. As such Top and DH5alpha should both be fine. Main differences are found in the methylation/restriction systems (and recombination apparatus). Link to comment Share on other sites More sharing options...
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