Hi there. I've been doing some biochemical tests on unknown microorganisms. They're from a UK kitchen, so nothing too exotic!

 

I was just wondering if I have sufficient evidence to convict;

 

1) a microorganism as Bacillus spp with the following:

 

Gram: positive rods, spores visible

Catalase: positive

Oxidase: negative

MacConkey: negative

 

2) a microorganism as Pseudomonas spp with the following:

 

Gram: negative rods

Catalase: positive

Oxidase: positive

MacConkey: negative

Urease: negative

 

3) a microorganisms as Klebsiella spp with the following:

 

Gram: negative rods

Catalase: positive

Oxidase: negative

MacConkey: positive

Urease: positive

Indole: negative

ONPG: positive

 

and 4) a microorganisms as E. coli with the following:

 

Gram: negative rods

Catalase: positive

Oxidase: negative

MacConkey: positive

Urease: negative

Indole: positive

ONPG: positive

 

Thanks in advance!

 

:)

Probably all good bets but not enough info to be assured.

#1 - might also be Geobacillus or Brevibacterium

#2 - could be alot of Gram neg nonfermenters

#3 - good bet but could be another enteric - e.g. Enterobacter agglomerans

#4 - real gooc bet - but could be E. agglomerans and some of the other Escherichia sp.

 

 

if you have singe islates - describe as "sp." - "spp." is plural.

Probably all good bets but not enough info to be assured.

#1 - might also be Geobacillus or Brevibacterium

#2 - could be alot of Gram neg nonfermenters

#3 - good bet but could be another enteric - e.g. Enterobacter agglomerans

#4 - real gooc bet - but could be E. agglomerans and some of the other Escherichia sp.

 

 

if you have singe islates - describe as "sp." - "spp." is plural.

 

Hi, thanks for your suggestions.

 

Agreed, I may need some more results to positively identify. For the Pseudomonas, as it is from the kitchen, from tap water, I thought this was the most likely organism.

 

For the others, yes there are a few alternatives. I'll have to look further into other tests I can do.

 

Thanks again

Bet you're right sbout the pseudomonad - were colony morphology, smelll and pigmentation consistent with P. aeruginosa?

Bet you're right sbout the pseudomonad - were colony morphology, smelll and pigmentation consistent with P. aeruginosa?

 

Hi there.

 

Colonies were a creamy white colour, about 1-2 mm in diameter on blood agar. To be honest, I can't remember what it smelt like and didn't note that down.

So P. aeruginosa is very unlikely.

 

This is what they looked like (picture from google images).

Edited April 17, 201213 yr by ozric