Hi I wondering if someone could help me.

 

 

If a plasmid that is in a cell has a specific type of RNA promotor and sits besides a Reporter Gene (but does not contain the gene for RNA polymerase for that promotor) and the cell it is in has the RNA polymerase gene in its chromosomal DNA is there a specific name for the process in which after the production of the RNA poylmerase has been induced and completed how does the RNA polymerase travel to the plasmid to bind to its promoter in order to create mRNA that are used to express the Reporter Gene.

 

Assuming that I have the right idea, what would this process be called?

 

Since I am referring to a bacteria cell and not have a nucleus I assume it doesn't matter where in the cell this takes place.

Edited April 5, 201213 yr by Really Lost

RNA polymerases are not specific to promotors. The strength of binding to certain promotors is mediated by transcription factors (including sigma factors).

How about a T7 RNA Polymerase and Promoter?

T7 RNA polymerase is a phage enzyme. They specifically deliver to the cell to synthesize their own proteins. Plasmids rely on their host machinery for transcription. They too, however, provide genes for their own replication.

 

If they do not possess the right promotors, the transcription rate is going to be very low (if transcribed at all).