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Calculating sizes of PCR products

Gamewizard
in Homework Help

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Hi

 

How can I using a 100bp ladder calculate the sizes of PCR products obtained with a 3' primer ?

 

 

Can someone please help me really stuck on this

  • Author

Do you know what a ladder is for?

 

 

To calculate the sizes of DNA fragments ? but how by plotting a calibration graph of fragments against distance migrated ? im not sure

  • Author

Yes, precisely. You have to think using semi-log scale, though (just plot it and you should see).

 

 

Okay, is it alright to use a semi-log paper with 3 cycles on the y axis and 1 cycle (upto 10) on the x axis? or does it have to be equal cycles on both x and y axis.

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