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Sanger method sequencing

ohoneo
in Homework Help

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You sequence the following DNA fragment: ACT GGG CAG AAG CGG GAA CTT TGC AGA ACT G GC TGG CCT CAG AG C AGG GA) by Sanger method (dideoxy sequencing) using primer [32P]-5’- TCT GAG GCC AGC CAG TTC TGC AAA GTT C. a) Predict the band pattern after electrophoretic analysis of the products of primer elongation. b) Due to an experimental mistake you forgot to add dATP in all four reaction mixtures. How does the band pattern change?

 

This is the problem I was giving. I worked on it for awhile and got nowhere really. I think that I don't understand what the question is asking me. How does the primer affect the band pattern?

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hint - a di-deoxy primer prevents elongation

 

So since we are using a di-deoxy primer, there's no elongation, which means there's no elongation products?

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