ohoneo

Hi all, I hope this is the right forum. I'm currently in a biochemistry class in my college and while I'm enjoying it, I'm finding that some of the practice problems prove rather difficult. Especially because it's so hard to find additional help on the Internet for them! I'm stuck on a problem, that I've copied and pasted below, along with my attempt to solve it: A primary structure of tRNA(Val) is pGGU UUC GUm1G GUC (psi)AG DGG DDA UGG CA(psi) CUG C(psi)U IAC ACG CAG AAC m7GDC m5CCC AGT (psi)CG m1AUC CUG GGC GAA AUC ACC A . Draw the secondary structure of tRNA indicate acceptor stem, D loop, T(psi)C loop, anticodon loop, variable loop, and CCA terminus. Draw the full chemical structures of the nucleotides m1G, m7G, m5C, I, psi, D. My attempt: tRNA I know I haven't drawn the full chemical structures, that's not my issue (not yet anyway!) My issue is that I'm really not sure about what I'm doing, and there's nothing in my textbook to help me, other than saying that tRNA has an acceptor stem, D, loop, T(psi)C loop, anticodon loop, variable loop, and CCA tail and how many nucleotides are in those sections. When drawing the secondary structure, I drew the nucleotides from left to right (starting with the acceptor stem, and then going left to the D arm). However, I had nucleotides from the primary structure left over, and I wasn't even sure if I was proceeding in the right direction. Also, I wasn't entirely sure if the modifications's subscripts referred to a specific modification or the location of it. Any help is much appreciated!

So since we are using a di-deoxy primer, there's no elongation, which means there's no elongation products?

You sequence the following DNA fragment: ACT GGG CAG AAG CGG GAA CTT TGC AGA ACT G GC TGG CCT CAG AG C AGG GA) by Sanger method (dideoxy sequencing) using primer [32P]-5’- TCT GAG GCC AGC CAG TTC TGC AAA GTT C. a) Predict the band pattern after electrophoretic analysis of the products of primer elongation. b) Due to an experimental mistake you forgot to add dATP in all four reaction mixtures. How does the band pattern change? This is the problem I was giving. I worked on it for awhile and got nowhere really. I think that I don't understand what the question is asking me. How does the primer affect the band pattern?