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The mechanism of GFP labeling

lukasbecker
in Homework Help

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I am trying to see if the GFP protein is used with anti-bodies to label the proteins OR something else. It says GFP can be fused to any protein of interest using standard methods of recombinant DNA. I guess that means that an antibody isn't necessary but...what is a standard method of recombinant DNA? HELP!

The fusion is done on the DNA level. I.e. you basically attach the gfp gene in-frame to your protein of interest, for example (details vary depending on what you want to get at the end).

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