I work on differentiation of stem cells to chondrocytes. I used pellet culture system for that.

now I want to do gag assay.

but I do not know how to digest this pellet and then use papain digestion solution.

and I have a another question about which concentrate of papain in suitable for gag assay.

thanks .

Azadeh

What's the pellet of?

The pellet culture system is a common model for chondrocytes differentiation from stem cells.

 

 

May bad... really don't know honestly, but your work sounds intriguing.

Hi Azzadeh,

 

I am doing my master's thesis within few days. I also did GAG assay as a marker for chondrogenic differentiation, but the problem is, I cannot find anything to support such claim. Could you please mention anything about how is GAG assay a marker for chondrogenic expression ?

 

Thanks in advance, and looking forward to hear from you and perhaps share my experience in this regard.

 

Greets

G