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Farnesylated proteins on a western blot. urgent!

futureless
in Biochemistry and Molecular Biology

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Hi, does anyone know if farnesylation could affect the mobility of a protein in a western blot? In particular, could farnesylated and unfarnesylated forn a doublet for a certain protein? or does farnesylation not show up, and the two forms would just migrate the same distance? help is very much appreciated, thanks in advance

:)

Well depends on the resolution and type of the gel as well as size of the protein. If you are in a very well resolved range it could be possible to see a shift.

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