Amela Posted April 16, 2011 Share Posted April 16, 2011 Hello there, I wonder if the FIX/PERM solution used during the Intra Cellular staining Could remove or kill red blood cells that could be present in the sample??? Thank you Link to comment Share on other sites More sharing options...
Bobr Posted April 16, 2011 Share Posted April 16, 2011 I cannot see the reason why it should, but I may be wrong. In that case, I would like to know why Link to comment Share on other sites More sharing options...
Amela Posted April 17, 2011 Author Share Posted April 17, 2011 The Permeabilisation solution that allows the antibodies to enter the cells could affect the integrity of the membrane and thus RBC may die cos of that, I was just wondering if the fixation solution could prevent this?? Some of my colleagues in the lab noticed that after ICS experiment RBC are reduced..... Link to comment Share on other sites More sharing options...
CharonY Posted April 19, 2011 Share Posted April 19, 2011 (edited) Basically all permeabilization and fixation methods put stress on cells. The composition of the solution and the resilience of the cells varies somewhat, though. Edited April 19, 2011 by CharonY Link to comment Share on other sites More sharing options...
Amela Posted April 23, 2011 Author Share Posted April 23, 2011 Ok, Thank you, Then any tips to get rid of the RBC that persist in the PBMC solution after Blood separation with Ficoll gradient??? Link to comment Share on other sites More sharing options...
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