Jump to content

Possible Mechanism for this reaction

Overbearingminds
in Organic Chemistry

Featured Replies

To make a long story short E1 (a protein) reacts with Ubiquitin (another protein) and covalently bonds via a cysteine residue....what type of reaction mechanism are we looking at here? The reaction is ATP dependent if it helps.

 

Thanks a lot in advance.

The ubiquitin has a carboxylic acid which is activated by ATP with a Mg2+ ion that helps activate the molecule. The activated phosphoester is attacked by the thiol of the E1 enzyme. As far as I can see, thats what you've asked for.

 

http://en.wikipedia.org/wiki/Ubiquitin

 

There is alot of information on this webpage about the mechanisms if you want.

ubiq.pdf

No problemo. Im not 100% sure of the mechanism, however thioester bonds are normally formed in this manor (i.e. in polyketide synthase enzymes)

Archived

This topic is now archived and is closed to further replies.

Go to topic listing

Important Information

We have placed cookies on your device to help make this website better. You can adjust your cookie settings, otherwise we'll assume you're okay to continue.

Configure browser push notifications
Chrome (Android)
  1. Tap the lock icon next to the address bar.
  2. Tap Permissions → Notifications.
  3. Adjust your preference.
Chrome (Desktop)
  1. Click the padlock icon in the address bar.
  2. Select Site settings.
  3. Find Notifications and adjust your preference.