Gamewizard Posted November 27, 2010 Share Posted November 27, 2010 Hi does anyone know how to assess the purity of the DNA sample, the 260/280 ratio for pure DNA is 1.8- 2.0 ? I extracted the DNA and my concentration was ng/ul 45.1 and the optical density was 2.06. please help me solve this Link to comment Share on other sites More sharing options...
CharonY Posted November 29, 2010 Share Posted November 29, 2010 The 260/280 ratio is based on the absorbance of the sample measured at the indicated wavelength. On OD of 2 is rather odd unless you factored dilutions in. Most photometers are unreliable with values near 1. Link to comment Share on other sites More sharing options...
Greippi Posted December 10, 2010 Share Posted December 10, 2010 (edited) An OD of 2.06 is MAD. I'm pretty sure your spectrophotometer will only be accurate below 1. You can't make sensible calculations with 2.06. Dilute your sample 1 in 10 and read again. EDIT: I'm sorry, CharonY pretty much said this. Also didn't notice how old the thread was. Edited December 10, 2010 by Greippi Link to comment Share on other sites More sharing options...
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