fengduhua Posted May 12, 2010 Share Posted May 12, 2010 1 to 4 are arabidopsis leaf ,5 to 8 are arabidopsis root why there are so many bands in leaf tissue,and what is the band in front of 28s rRNA Link to comment Share on other sites More sharing options...
Greippi Posted May 12, 2010 Share Posted May 12, 2010 Eeek, your picture isn't showing up. Link to comment Share on other sites More sharing options...
CharonY Posted May 12, 2010 Share Posted May 12, 2010 Depending on your isolation additional bands are to be expected, especially in very active tissues. For quality control one usually would check the 28/18s ratio. Very high MW bands can also be co-isolated DNA. Link to comment Share on other sites More sharing options...
fengduhua Posted May 13, 2010 Author Share Posted May 13, 2010 Depending on your isolation additional bands are to be expected, especially in very active tissues. For quality control one usually would check the 28/18s ratio. Very high MW bands can also be co-isolated DNA. thanks for your reply, does it have such a possibility that the chlorophy body rRNA and chondriosome rRNA are extracted from leaf tissue meanwhile Link to comment Share on other sites More sharing options...
CharonY Posted May 17, 2010 Share Posted May 17, 2010 It depends precisely on what you are seeing and your extraction protocol. Are the additional bands on the height of the 16S rRNA? Link to comment Share on other sites More sharing options...
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