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Thermodynamic Box

fid786
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A myoglobin had the protonation of a histidine residue coupled to the oxidation of a heme. The histidine had a pKA of 6.0 when the heme is oxidized and 7.1 when the heme is reduced. At pH 9.5, the reduction potential of the heme is +275 mV vs NHE.

 

(a) Draw the thermodynamic box that describes this system

 

(b) Predict the reduction potential at pH 3.

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I could not do this problem. I was having problems understanding how to make the box.

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