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electrophoresis gels

curtis123
in Biochemistry and Molecular Biology

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I am having a hard time understanding how to interpret electrophoresis gels. I have spent a lot of time looking into this and need some help.

 

Why do double digests sometime create one fragment on the gel? I thought that there should be more fragments since the DNA is being cut twice?!

It depends on a) the form of the DNA, b)how many restriction sites for the given enzyme are present on the DNA molecule c) where the restrictions sites are and d) the resolution of the gel (i.e. which sizes are finally visible).

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