Abchirk Posted January 23, 2010 Share Posted January 23, 2010 Hey I have searched in the internet, but I didn't find anything related to that. I found how to extract the hot substances like gingerol. But I didn't find anything for ascorbic acid. So does anyone has an idea? I want to quantify the amount of vitamin C in ginger. It is for a project work at school. Any suggestion is welcome! Link to comment Share on other sites More sharing options...
Horza2002 Posted January 26, 2010 Share Posted January 26, 2010 Seeing as Vit C is an acid, you should be able to rextracted it by washing with an basic solution followed by reacidifying it Link to comment Share on other sites More sharing options...
Abchirk Posted January 26, 2010 Author Share Posted January 26, 2010 Yeah and then maybe titration, but we have to use modern analytic methods like HPLC etc. I found some papers... Does anyone has free access to papers like this: - quantifying via GC - it would be very appreciated. I already got this one: - quantifying via HPLC -, but I guess our HPLC doesn't have such column. Merged post follows: Consecutive posts mergedhm well, I got all papers now... I have decided to use HPLC with a gradient (oH-level ~2) Because you need something which prevents the ascorbic acid of degrading to dehydroascorbic acid. You can achieve this with substances which block the oxidizing of Ascorbicacid or a acid like metaphyophoric acid which also safes the ascorbic acid. Link to comment Share on other sites More sharing options...
John Cuthber Posted January 26, 2010 Share Posted January 26, 2010 Since vitamin C is very soluble in water there's nothing much to gain by using a base to extract it. Also, it is less likely to be oxidised by the air if it is in neutral or acid solution. (BTW, a lot of the column manufacturers supply "application notes" for their columns. It's blatant advertising, but it's a good way to find out about analytical methods, on-line, for free.) Link to comment Share on other sites More sharing options...
Abchirk Posted January 31, 2010 Author Share Posted January 31, 2010 (BTW, a lot of the column manufacturers supply "application notes" for their columns. It's blatant advertising, but it's a good way to find out about analytical methods, on-line, for free.) Yes, I know I was a bit in hurry... But I asked my teacher now and he said that I could do it with pH=2 also on the column, but I have to re-neutralize the column every day... Thanks for your help! Link to comment Share on other sites More sharing options...
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