Gretz26 Posted November 27, 2009 Share Posted November 27, 2009 Why is there a change in pH after freeze drying Goat IgG with ammonium bicarbonate buffer but no pH change for rabbit IgG when freeze dried the same? Thanks Link to comment Share on other sites More sharing options...
dttom Posted November 28, 2009 Share Posted November 28, 2009 Could you show the reference of this result? Link to comment Share on other sites More sharing options...
CharonY Posted November 28, 2009 Share Posted November 28, 2009 How do you determine the pH of a dried substance? Link to comment Share on other sites More sharing options...
Gretz26 Posted December 3, 2009 Author Share Posted December 3, 2009 Hi Sorry no reference that's what I need though. Personnal experience from friends working with the stuff. Merged post follows: Consecutive posts mergedTest the pH of the F/D by re-dissolving in MQ water. Link to comment Share on other sites More sharing options...
CharonY Posted December 4, 2009 Share Posted December 4, 2009 You should check whether the effect is reproducible. During evaporation CO2 and hence, buffer capacity gets lost. However, the proteins themselves are afaik not different enough to result in different pH (I would have to check the sequence to verify that, though). I would rather assume that it is essentially a problem of a non-buffered solution. Small difference of additives or even protein concentration can shift the pH easily. Link to comment Share on other sites More sharing options...
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