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protocol for S.aureus transformation

Jen_23
in Biochemistry and Molecular Biology

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Hi All,

 

does anyone have a protocol which they have used to successfully transform a plasmid into a staph species?

I've tried 4 protocols in duplicate on 8 strains and I'm getting nowhere!!

 

Thanks

 

Jen

Were the protocols you used specifically for S. aureus? AFAIK there are a few out there. If those do not work I would start troubleshooting the process than yet try another one (doing it in duplicate is not really troubleshooting, but rather counts as doing a backup).

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