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Brain Heart Infusion Broths and Coagulase test

Hersch
in Microbiology and Immunology

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gday folks. Im looking to confirm S.aureus in a food sample and so far ive inoculated a GC broth (+), inoculated BPA from the positive GC broth and about to inoculate BHIB ready to perform a coagulase test. My question is what is the reason for inoculating BHIB prior to doing a coagulase test. thanks!

 

hope that makes sense..

 

hersch

If you are asking whether inoculation of in BHis is a prerequisite for a coagulation test, then the answer is no. However it is easier to have a liquid culture to do the test as you can easier add a defined amount of your culture (from BHis in this case).

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