Tris is a widely used buffer in biochemistry and molecular biology research. Its molecular weight is 121.14g/mol. how many gram of Tris you need to male 1L of 1 M stock solution ? How many mL of water you need to make a 0.1 M Tris solution from 10 mL of 1M stock solution? If the 1 M Tris stock solution is labeled as 1000 X , what is the working concentration ? How many mL of stock solution you need to make 1 L of working solution?

 

Please help me with this!!!!!

gm of tris = 1L x 1M x FW (121.14) = 121.14 gm

if u have 1M solution and have to make 0.1M it means you have to dilute it 10 times. (1M/0.1M = 10 dilution factor) 1 ml of your stock(1M) and 9 ml of water to get 0.1M.

1000X you have to dilute it 1000 times ( not really feasible to have 1000X solution of tris)

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