fibonacci Posted July 15, 2007 Share Posted July 15, 2007 my supervisor said that when sequencing EST, we juz sequence it 1 time and juz 1 strand...if i'm not mistaken from 5'-end... can anyone tell me why???? i'm juz curious.... :confused: Link to comment Share on other sites More sharing options...
CharonY Posted July 15, 2007 Share Posted July 15, 2007 If your question is why you only sequence one strand, I cannot answer that (I'd always take both to reduce errors). Regarding the sequencing direction: You essentially got two possibities 3'->5' or 5'->3'. Now think about how Sanger sequencing works. (In the end it is only a question where you put the primer, though). Link to comment Share on other sites More sharing options...
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