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Est...

fibonacci
in Biochemistry and Molecular Biology

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my supervisor said that when sequencing EST, we juz sequence it 1 time and juz 1 strand...if i'm not mistaken from 5'-end...

 

can anyone tell me why????

i'm juz curious....:confused: :confused: :confused:

If your question is why you only sequence one strand, I cannot answer that (I'd always take both to reduce errors).

Regarding the sequencing direction:

You essentially got two possibities 3'->5' or 5'->3'. Now think about how Sanger sequencing works.

(In the end it is only a question where you put the primer, though).

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