KFC Posted March 20, 2007 Share Posted March 20, 2007 Can someone tell me in depth, the process in E. Coli Plasmid Cloning and the chemical that are used"? Link to comment Share on other sites More sharing options...
ecoli Posted March 20, 2007 Share Posted March 20, 2007 there's not one way to do it... what are you interested in? Link to comment Share on other sites More sharing options...
KFC Posted March 20, 2007 Author Share Posted March 20, 2007 Transfering a gene from a Eukartoyic cell to a E. coli Bacterium using a Plasmid with an AMPr gene using ecoRI, CaCl2, and DNA ligase. I'm not sure what the process is named. Link to comment Share on other sites More sharing options...
ecoli Posted March 20, 2007 Share Posted March 20, 2007 look up the pGem easy vector system by Promega. Link to comment Share on other sites More sharing options...
KFC Posted March 20, 2007 Author Share Posted March 20, 2007 How much times harder is this then making Nitric acid? Link to comment Share on other sites More sharing options...
insane_alien Posted March 20, 2007 Share Posted March 20, 2007 !!!! you think transfering a gene is going to be comparable with nitric acid? are you on planet earth? Link to comment Share on other sites More sharing options...
KFC Posted March 20, 2007 Author Share Posted March 20, 2007 No, I'm at your local KFC Ecoli, Is that where i get the chemical?. Link to comment Share on other sites More sharing options...
KFC Posted March 20, 2007 Author Share Posted March 20, 2007 (Sorry empty post) Link to comment Share on other sites More sharing options...
ecoli Posted March 21, 2007 Share Posted March 21, 2007 No, I'm at your local KFC Ecoli, Is that where i get the chemical?. look on the Promega website. They should have the product(s) and protocol. If not, post here again, and I can help you further. Link to comment Share on other sites More sharing options...
KFC Posted March 21, 2007 Author Share Posted March 21, 2007 Is this it Link to comment Share on other sites More sharing options...
ecoli Posted March 22, 2007 Share Posted March 22, 2007 no... that seems to be about PCR, you want plasmid cloning. Start here: http://www.promega.com/search/Default.aspx?query=pGem+easy+vector&x=0&y=0 Link to comment Share on other sites More sharing options...
KFC Posted March 22, 2007 Author Share Posted March 22, 2007 Cool Thanks, I was wondering if the proteins made from the Plasmid are they moved out of the cell or Kept in the E. coli. Link to comment Share on other sites More sharing options...
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