Jump to content

E. Coli Plasmid Cloning

KFC
in Biology

Featured Replies

Can someone tell me in depth, the process in E. Coli Plasmid Cloning and the chemical that are used"?

  • Author

Transfering a gene from a Eukartoyic cell to a E. coli Bacterium using a Plasmid with an AMPr gene using ecoRI, CaCl2, and DNA ligase. I'm not sure what the process is named.

  • Author

How much times harder is this then making Nitric acid?

!!!! you think transfering a gene is going to be comparable with nitric acid?

 

are you on planet earth?

  • Author

No, I'm at your local KFC

 

Ecoli, Is that where i get the chemical?.

  • Author

(Sorry empty post)

No, I'm at your local KFC

 

Ecoli, Is that where i get the chemical?.

 

look on the Promega website. They should have the product(s) and protocol. If not, post here again, and I can help you further.

  • Author

Is this it

  • Author

Cool Thanks,

 

I was wondering if the proteins made from the Plasmid are they moved out of the cell or Kept in the E. coli.

Archived

This topic is now archived and is closed to further replies.

Go to topic listing

Important Information

We have placed cookies on your device to help make this website better. You can adjust your cookie settings, otherwise we'll assume you're okay to continue.

Configure browser push notifications
Chrome (Android)
  1. Tap the lock icon next to the address bar.
  2. Tap Permissions → Notifications.
  3. Adjust your preference.
Chrome (Desktop)
  1. Click the padlock icon in the address bar.
  2. Select Site settings.
  3. Find Notifications and adjust your preference.