Materials: 6M HCl and protein solution

 

Condition : nitrogen gas atmosphere (to prevent oxidation), 150 degree celcius for 65 mins.

 

What apparatus or instruments to be used to do this hydrolysis?

western blots are for proteins, but if your breaking them down, I don't know how that would work. Amino acid chromotography maybe?

Yeah with those conditions you'll just end up with amino acids.

 

Well you'll definatley need some acid resistant pressurized container.

If I perform classical acid hydrolysis using same materials, the hydrolysis temperature would be 110 degree celcius and time frame is 24 hours and above.

 

Can I perform the classical acid hydrolysis in separate days (3 days, 8 hours per day)?