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sasu

rbc lysis

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We use ammonium chloride with Tris base as the buffer. It works just as well as the commercial sol'ns and is a thousand times cheaper.

 

p.s. - Rakuenso, RBC lysis is used to remove red blood cells from cell suspensions as they interfere with flow cytometric analysis and tissue culture.

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what are you trying to extract? obviously not DNA.

 

i want to separate rbc & wbc by chromatography

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what are you trying to extract? obviously not DNA.

 

i want to separate rbc & wbc by chromatography

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ohh i see

 

I thought you wanted to lyse individual RBCs and analyze its plasma contents

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one simple que . we are using 5% dextrose & 0.9% NaCl solution as isotonic solution. but osmotic pressure of 5% dextrose 6.7 atm & 0.9% NaCl 3.7 atm. then why rbc get breaked if we use 3.7 atm of dextrose solution?

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No, plain water would lyse all the cells. You need a certain osmotic pressure to lyse the RBCs preferentially.

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