can anybody tell me goood method for rbc lysis:embarass:

what are you trying to extract? obviously not DNA.

We use ammonium chloride with Tris base as the buffer. It works just as well as the commercial sol'ns and is a thousand times cheaper.

 

p.s. - Rakuenso, RBC lysis is used to remove red blood cells from cell suspensions as they interfere with flow cytometric analysis and tissue culture.

what are you trying to extract? obviously not DNA.

 

i want to separate rbc & wbc by chromatography

what are you trying to extract? obviously not DNA.

 

i want to separate rbc & wbc by chromatography

ohh i see

 

I thought you wanted to lyse individual RBCs and analyze its plasma contents

one simple que . we are using 5% dextrose & 0.9% NaCl solution as isotonic solution. but osmotic pressure of 5% dextrose 6.7 atm & 0.9% NaCl 3.7 atm. then why rbc get breaked if we use 3.7 atm of dextrose solution?

search for ACK lysis buffer.

Wouldn't plain water do the trick?

No, plain water would lyse all the cells. You need a certain osmotic pressure to lyse the RBCs preferentially.