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Biphasic Amplification in qPCR

stubbsey12
in Genetics

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image.thumb.png.697e43c3136c33cd9a1d096fdf1f4375.png

Is anyone able to explain this biphasic amplification that I've been seeing in my positive controls (Green curve)?

 

For reference, this is using TaqMan probe technology, whose targets are simultaneously nsp16 and orf1ab (COVID-19).

 

Please ask for further explanation! I cannot find any resources out there which can explain this..

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