Jump to content

DNA extraction from bird feathers using magnetic bead-based method


Tania Mahmood
 Share

Recommended Posts

Hi, anyone please make me clear if it is possible to extract DNA from bird feathers by magnetic bead-based DNA extraction method ? If any kind of literature or protocol is available on it then kindly share the link also.



Thanks in advance.

Link to comment
Share on other sites

Not my field of expertise, but fundamentally the purification step (e.g. columns vs liquid vs magnetic bead extraction) is less critical as there are many variations to improve yield or other critical parameters, depending how you scale them. 

Often the issues before that step, such as how fresh the specimens are and the lysis steps which have a higher impact. And how critical it is, also depends on the precise workflow after isolation. If you just want to do a PCR e.g. on mtDNA, it is easier than getting high quality DNA for whole genome sequencing, for example.

From what I can see it is fairly straightforward for fresh feathers, but if you are dealing with older ones, you might want to look at protocols that specifically refer to molted feathers.

Link to comment
Share on other sites

Create an account or sign in to comment

You need to be a member in order to leave a comment

Create an account

Sign up for a new account in our community. It's easy!

Register a new account

Sign in

Already have an account? Sign in here.

Sign In Now
 Share

×
×
  • Create New...

Important Information

We have placed cookies on your device to help make this website better. You can adjust your cookie settings, otherwise we'll assume you're okay to continue.