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How you will prepare the following solutions:

Solution 1 (500 mL; pH 8.5)

1% Tryptone + 0.5% yeast extract + 200 mMNaCl

Solution 2 (500 mL; pH 8.8)

50 mM glucose + 10 mM EDTA + 25 mMTris (pH 8.0)

I'd probably ask a student named Hussain to do it for me. That way, he could use his time to work through it and learn something instead of me doing it for him. 

More here: 

 

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