northernlad2690 Posted May 7, 2018 Share Posted May 7, 2018 Hello, I'm just wanting someone to give me a little bit of guidance on the following conversion, i'm trying to convert my reading of umol/min/umol to umol/min/mg- i think i have the correct answer but i need my method validated if someone could possibly help? i have a spectrophotometric assay of a purified recombinant enzyme, Spy057, against p-nitrophenyl N-acetyl-β-glucosaminide substrate (total reaction volume, 2 mL; volume of Spy05783 added, 50 µL): - I have calculated the umol/min/umol using beer lamberts law to 289.63 - the Mr of spy057 is 43507g/mol - i then calculated that there is 43.507mg/umol - there is 1.899 x 10E-4 umol added reaction volume so using this i then calculated 43.507x(1.899E-4) = 8.272 x 10E-3 using data obtained previously i knew that the umol/min of p-nitrophenol was 0.055 so this was input into the final equation 0.055/8.272 x 10E-3 giving me 6.657umol/min/mg does this look correct, ive done everything to try and find a protocol but cant find anything Link to comment Share on other sites More sharing options...
studiot Posted May 7, 2018 Share Posted May 7, 2018 Duplicate thread Link to comment Share on other sites More sharing options...
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