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Enzyme calculation

John.matheson
in Biochemistry and Molecular Biology

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Hi All,

 

Looking for some advice.

 

If I have a protease liquid enzyme of unknown activity how do I calculate activity in U/ml using a colorimteric substrate?

 

All best,

 

Niall

Assuming you have an spectrophotometer, you make a linear standard with a defined product and measure optical density at the optimum wavelength.

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