Mzebra

Hi, My names Aaron, I am currently carrying out a project (as part of an MRes) that is looking to identify a promoter region of the Pax7 gene in the cichlid fish Metriaclima zebra. The reason for this is that Pax7 in certain cichlid fish is thought to be responsible to some degree for a colour polymorphism (orange-blotch). Although Pax7 does not show phenotype associated sequence differences it is thought a promoter region upstream might. I am hoping to find a candidate region through bioinformatics (an area I am totally unfamiliar with - as my last piece of work was a behavioral based study) which I will then look at more closely by designing the appropriate primers etc etc (again I'm really quite uncomfortable with molecular biology)