Shyenne2411 Posted May 23, 2016 Share Posted May 23, 2016 Hi all, I would like to ask if you know about molecular technique to quantificate intracellular bacteria from biopsy samples. Flow cytometry require labing of bacteria, which is not possible if you are testing biopsies. Another option which popped into my mind is qPCR - however how would you overcome with possible DNA fragments in sample? Is there any other technique you would recommend? I will by really thankful for any help. Link to comment Share on other sites More sharing options...
CharonY Posted May 23, 2016 Share Posted May 23, 2016 Usually, you use microscopy. Link to comment Share on other sites More sharing options...
Shyenne2411 Posted May 23, 2016 Author Share Posted May 23, 2016 Thank you, I´ve read about that, However as I understand, you use microscopy for detection, I cant imagine how would smb count the bacteria. Isnt there any easier amd quicker possibility? Link to comment Share on other sites More sharing options...
Recommended Posts
Create an account or sign in to comment
You need to be a member in order to leave a comment
Create an account
Sign up for a new account in our community. It's easy!
Register a new accountSign in
Already have an account? Sign in here.
Sign In Now