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Transcription of DNA which goes in direction 5'-3'


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Hi all :)

I have a question concerning transcription

Problem goes like this: If RNA pol transcribes DNA from right to left, write newly sinthetised mRNA along with orientations.

This is DNA:

5'-ATATCCCTAATCCG-3'

3'-TATAGGGATTAGGC-5'

 

I understand that RNA pol can't go from left to right in direction 3'-5', thus we are talking about transcribing DNA which goes in 5-3 direction.

So this is what I got a mRNA, and final solution to this problem:

 

3'-UAU AGG GAU UAG GC 5'

Is this correct?
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Unfortunately no. 5'-->3' is the coding strand, while 3'-->5' is the template strand. RNA pol binds to the template strand, and transcribes in the complementary 5'-->3' direction, so the resultant mRNA reads 5'-->3', the same as the coding strand (which is why the coding strand is so named). The mRNA should read the same as the coding strand, except U subbed for T.

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