Nubellybus Posted February 3, 2016 Share Posted February 3, 2016 Hi all, I've a query about what stage fluorescence is measured at in a digital PCR reaction. Is it continuous or only at the end of the reaction? i thought the former, but I'm after reading a paper stating the latter in a matter of fact way. Can it be both?? Thanks guys!!! Link to comment Share on other sites More sharing options...
NucleotidalWave22 Posted February 4, 2016 Share Posted February 4, 2016 It measures before, during and after in order to quantify the rate in which the PCR has multiplied the sample. 1 Link to comment Share on other sites More sharing options...
CharonY Posted February 5, 2016 Share Posted February 5, 2016 Digital PCR pretty much only refers to the partitioning of the template prior to the reaction. As it can have many different applications, there can be differences when measurements occur. Even in cases of qPCR it is not necessary to monitor continuously as in standard approaches. Rather, the amount of negative to positive partitions and Poisson statistics can be used to calculate amount. In this case an endpoint measurement would suffice. Link to comment Share on other sites More sharing options...
Recommended Posts
Create an account or sign in to comment
You need to be a member in order to leave a comment
Create an account
Sign up for a new account in our community. It's easy!
Register a new accountSign in
Already have an account? Sign in here.
Sign In Now