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Help I need to make LB Agar Dish (I need it within 2 days of posting this)


wpan2002

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Ok soo hi i am kinda in a rush since i dont know how to make a LB agar dish (Made for growing bacteria like the ones on your keyboard that your maybe responding to this post with) I completely forgot to ask the seller i bought these Petri Dishes How to make them and this is going to help me with my homework i am trying to grow bacteria from a agar dish and seeing what chemicals will kill them more efficiently so i am not asking for a answer for my homework btw

 

The Reason i am in a rush because today i bought the dish some (Pyrex 3160-100 Dish, Culture, Petri, Complete, 100x10mm 7740 Glass 12 EA/Pack for Laboratory Use Only) Completely no Clue were the seller bought it from but is brand new box made in Germany now i need to make the stuff for the inside but i dont know how to make it these Dishes are Glass soo i also have completely no clue how to sanitize them

 

I need it to grow the bacteria quick and i have one of those heaters that heat the room to a certain temp so i guess it would help it i hear LB Agar Dishes are good at making these bacteria grow (The ones on your Keyboard) soo i wonder if someone can give me instruction and were to buy the stuff since i will need to go buy it locally since i will need it 2 days after making this post

 

If you can I will be ok traveling to Burnaby, Surrey, Coquitlam, Vancouver (I cant go to Vancouver Island By the way) (Canada, British Columbia)

 

Please Tell me to Tools and Materials i will need

Things i have already:

- Six Fortune Agar Agar Strips (it says 40g on the pack)

- Pyrex Lab ware 100x10mm Glass Petri Dish

- A Portable Stove if i will need to do it outside ._.

- Microscope (Soo much types my school is providing :D)

- Heater

 

I want to make 12 of these Dishes

 

I have no idea what the heck i am doing so please help me out from

Sanitizing the Glass Petri Dishes to Starting The Bacterial Growing Cycle

 

(Also Tell the Cautions Please)

 

Tell me also were da heck is full of nasty bad bacterial (My Idea is my sister's hair, Phone, Keyboard, Laptop, Mouse)

 

 

Please and thanks my grades are counting on you

(Also to Remind you this is not doing my homework since i look at many parts of the internet and i have no clue and my homework is not Growing it is me killing it with chemicals)

(Also this is my less hope since i got banned on like 4 forums Since i was underage [i was asking people on Liquor Sites O.o])

 

Please make it as detailed as you can because i need as much help as i can get since i have no clue

(If i have to i know people who work at Simon Fraser University that maybe can help but i its hard to get hold of him)

 

Also i need to know were i can buy them because i was hunting for 2 Days trying to find petri dishes (Well Most of them dont want to sell it to me a 13Year old kid because i went to alot of liquor stores they kicked me out and threaten to call the cops so Brewing Stores are out of my reach) Which i dont want to go hunting for other again Which if i get help from you guys i can still have time to do homework and school Please and thanks for you all

 

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Sanitizing the Glass Petri Dishes to Starting The Bacterial Growing Cycle

 

 

Sterilize the Petri Dishes in an autoclave. Alternatively you can use a strong pressure cooker but this may not be designed to reach the required temp 250F for 15 minutes always read the manufacturer's instructions prior to doing so as it can be dangerous.

 

Tell me also were da heck is full of nasty bad bacterial (My Idea is my sister's hair, Phone, Keyboard, Laptop, Mouse)

 

All good places. I took mine from the school cafeteria.

 

I want to make 12 of these Dishes

Then you will have to make do with half an agar packet for each one.

 

Also i need to know were i can buy them because i was hunting for 2 Days trying to find petri dishes

 

Ebay

 

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Sterilize the Petri Dishes in an autoclave. Alternatively you can use a strong pressure cooker but this may not be designed to reach the required temp 250F for 15 minutes always read the manufacturer's instructions prior to doing so as it can be dangerous.

 

 

All good places. I took mine from the school cafeteria.

 

Then you will have to make do with half an agar packet for each one.

 

Ebay

 

how am i going to find the Agar

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