devaughn Posted March 14, 2014 Share Posted March 14, 2014 is there any similarities between enzyme linked immunosorbent (ELISA) and western blotting? Link to comment Share on other sites More sharing options...
CharonY Posted March 16, 2014 Share Posted March 16, 2014 Yes. Link to comment Share on other sites More sharing options...
zoe winslet Posted June 28, 2016 Share Posted June 28, 2016 Both ELISA and Western Blot are called indirect tests as they measure the immune system’s response to an infectious agent rather than looking for the components of the agent itself. Since ELISA detects HIV antibodies which the body starts to produce between 2-12 weeks after becoming infected with HIV, experts say that one should wait for at least 3 months after unprotected test to confirm for HIV AIDS. Western Blot is the most common method of testing to confirm positive results from ELISA test. Western Blot is used more as a confirmatory test as it is difficult to perform and requires high skills. Link to comment Share on other sites More sharing options...
CharonY Posted June 28, 2016 Share Posted June 28, 2016 Apart from the fact that this is a two-year old thread, it should be added that neither ELISA nor Western are exclusively used to measure antibodies. In fact they can (and often are) used to quantify anything that can be a antigen, including proteins other than immunoglobulins, peptides, hormones as well as other immunogenic biomolecules. Link to comment Share on other sites More sharing options...
Recommended Posts
Create an account or sign in to comment
You need to be a member in order to leave a comment
Create an account
Sign up for a new account in our community. It's easy!
Register a new accountSign in
Already have an account? Sign in here.
Sign In Now