sasu Posted October 6, 2005 Share Posted October 6, 2005 can anybody tell me goood method for rbc lysis:embarass: Link to comment Share on other sites More sharing options...
rakuenso Posted October 6, 2005 Share Posted October 6, 2005 what are you trying to extract? obviously not DNA. Link to comment Share on other sites More sharing options...
zyncod Posted October 6, 2005 Share Posted October 6, 2005 We use ammonium chloride with Tris base as the buffer. It works just as well as the commercial sol'ns and is a thousand times cheaper. p.s. - Rakuenso, RBC lysis is used to remove red blood cells from cell suspensions as they interfere with flow cytometric analysis and tissue culture. Link to comment Share on other sites More sharing options...
sasu Posted October 6, 2005 Author Share Posted October 6, 2005 what are you trying to extract? obviously not DNA. i want to separate rbc & wbc by chromatography Link to comment Share on other sites More sharing options...
sasu Posted October 6, 2005 Author Share Posted October 6, 2005 what are you trying to extract? obviously not DNA. i want to separate rbc & wbc by chromatography Link to comment Share on other sites More sharing options...
rakuenso Posted October 6, 2005 Share Posted October 6, 2005 ohh i see I thought you wanted to lyse individual RBCs and analyze its plasma contents Link to comment Share on other sites More sharing options...
sasu Posted October 12, 2005 Author Share Posted October 12, 2005 one simple que . we are using 5% dextrose & 0.9% NaCl solution as isotonic solution. but osmotic pressure of 5% dextrose 6.7 atm & 0.9% NaCl 3.7 atm. then why rbc get breaked if we use 3.7 atm of dextrose solution? Link to comment Share on other sites More sharing options...
Emily Posted October 12, 2005 Share Posted October 12, 2005 search for ACK lysis buffer. Link to comment Share on other sites More sharing options...
NPK Posted October 15, 2005 Share Posted October 15, 2005 Wouldn't plain water do the trick? Link to comment Share on other sites More sharing options...
zyncod Posted October 16, 2005 Share Posted October 16, 2005 No, plain water would lyse all the cells. You need a certain osmotic pressure to lyse the RBCs preferentially. Link to comment Share on other sites More sharing options...
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