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rbc lysis Rate Topic: -----

#1 6 October 2005 - 04:55 AM sasu 


Lepton
can anybody tell me goood method for rbc lysis:embarass:
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#2 6 October 2005 - 05:21 AM rakuenso 


Molecule
what are you trying to extract? obviously not DNA.
Quaero Veritas
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#3 6 October 2005 - 05:36 AM zyncod 


Atom
We use ammonium chloride with Tris base as the buffer. It works just as well as the commercial sol'ns and is a thousand times cheaper.

p.s. - Rakuenso, RBC lysis is used to remove red blood cells from cell suspensions as they interfere with flow cytometric analysis and tissue culture.
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#4 6 October 2005 - 05:37 AM sasu 


Lepton

rakuenso said:

what are you trying to extract? obviously not DNA.


i want to separate rbc & wbc by chromatography
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#5 6 October 2005 - 05:37 AM sasu 


Lepton

rakuenso said:

what are you trying to extract? obviously not DNA.


i want to separate rbc & wbc by chromatography
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#6 6 October 2005 - 05:42 AM rakuenso 


Molecule
ohh i see

I thought you wanted to lyse individual RBCs and analyze its plasma contents
Quaero Veritas
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#7 12 October 2005 - 09:02 AM sasu 


Lepton
one simple que . we are using 5% dextrose & 0.9% NaCl solution as isotonic solution. but osmotic pressure of 5% dextrose 6.7 atm & 0.9% NaCl 3.7 atm. then why rbc get breaked if we use 3.7 atm of dextrose solution?
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#8 12 October 2005 - 03:31 PM Emily 


Lepton
search for ACK lysis buffer.
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#9 15 October 2005 - 10:40 AM NPK 


Meson
Wouldn't plain water do the trick?
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#10 16 October 2005 - 09:46 PM zyncod 


Atom
No, plain water would lyse all the cells. You need a certain osmotic pressure to lyse the RBCs preferentially.
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