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How to find protein information?


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#1 shanglei1986

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Posted 20 January 2017 - 07:58 AM

For example, a kind of enzyme is characterized by the N-terminal Serine/Threonine kinase. And my question is how to find the kinase activity site, how to make the kinase dead with one site mutation without any reference.


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#2 CharonY

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Posted 20 January 2017 - 12:20 PM

There are several databases that can assist you in that, though of course without actual experimental verification they all will just be predictions based on sequence similarities. Uniprot is a general database that aggregates a lot of this information. If you want to implement something yourself you can look into protein family/domain databases such as pfam, prosite, etc. 

But again, usually you would knock out the whole domain, or at least a sizeable chunk of it. Figuring out the required minimum mutation to deactivate a given activity is quite a bit trickier.


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#3 shanglei1986

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Posted 24 January 2017 - 06:20 AM

There are several databases that can assist you in that, though of course without actual experimental verification they all will just be predictions based on sequence similarities. Uniprot is a general database that aggregates a lot of this information. If you want to implement something yourself you can look into protein family/domain databases such as pfam, prosite, etc. 

But again, usually you would knock out the whole domain, or at least a sizeable chunk of it. Figuring out the required minimum mutation to deactivate a given activity is quite a bit trickier.

Thanks for your reply


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