Hi! I have a problem with a fused protein. I want to know the optimal pH and temperature range (among other physical properties) of a fused protein, made of two mini-proteins. However, I'm still waiting for my DNA synthesis to arrive in order to produce it and I only have a few properties of the mini-proteins.

 

I do have my fused protein modelling and structure, but I cannot predict any of these parameters. Does anyone know about how can I predict (in the most accurate possible way) pH, temperature and isoelectric point of my fused protein without experimenting? Thank you!

It is possible to estimate the pI of any protein of known sequence. Just import the sequence into a pI calculation routine, such as the one at ExPASy.

You can use some software to predicate the basic information of your protein.

I think the following site may be useful for you：

https://en.wikipedia.org/wiki/List_of_protein_structure_prediction_software

 

Or you can seek help from some commercial company such as Predictprotein and Creative Biostructure.