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number of bacterial cells

Silvia_84
in Microbiology and Immunology

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Hi all,

 

I have to do PFGE on some strains of Klebsiella. It is the first time I do it and I am reading a protocol for the prepararation of agarose embedded bacterial DNA. The procedure says at a certain point to remove 5 X 108 cells (I suppose they refer to a bacterial suspension) for each ml of agarose plugs to be made. Can you explain me how you determine the volume of bacterial suspension corresponding to this amount of cells (5 X 108) and how is it calculated? I need to know it even for other protocols that say to take a specific number of cells and I did not understand how to convert this amount in volume.

 

I thank you very much.

 

Do you have a way to calculate cell concentration via automated or indirect counting (i.e. flow cytometry, coulter counter or CFU counting)?

 

~EE

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