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p-nitrophenyl phosphate/p-nitrofenol

ilke
in Homework Help

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Hi :)

We are working in the lab with enzym (serum),... and we have to make a p-nitrophenyl phosphate and a p-nitrophenol solution (10mM), but we have to solve it in HCl (1 mM), I can't figure out why? Because it's also soluble in water...

Other details: the pH is 10 (glycin buffer), we stop the reaction with 100 mM NaOH,..

Can someone help us?

Edited by ilke

It is difficult to be certain what your question is. Is the enzyme a phosphatase? Many phosphatases take para-nitrophenylphosphate as a substrate and produce para-nitrophenol as a product. Is the para-nitrophenylphosphate dissolved in 1 mM HCl. Or do you mean that the para-nitrophenol is dissolved in 1 mM HCl?

 

What do you know about the acid-base chemistry of para-nitrophenol? What do you know about the UV/VIS spectrum of this compound?

Edited by BabcockHall

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