sunny Posted February 11, 2013 Share Posted February 11, 2013 can any one help me on bacterial rnase activity assay? which is the bst method for it? Link to comment Share on other sites More sharing options...
BabcockHall Posted February 12, 2013 Share Posted February 12, 2013 (edited) What is the specificity of the enzyme from bacteria, as opposed to ribonuclease A, for example? Which assays have you considered so far? Edited February 12, 2013 by BabcockHall Link to comment Share on other sites More sharing options...
sunny Posted February 13, 2013 Author Share Posted February 13, 2013 helo i used kamekura and onishi method for rnase activity.briefly its steps r: buffer pH 7 enzyme .1ml rna 1mg incubation for 2 hr stop solution centrifuge then in end dilution of supernatent. in many other protocol more or less all steps are same but i cant get the logic related to dilutions. is it the necessary step or what??? as if i have the O.D 2.06 of test y i dilute it???? and if i dilute at which dilution i used??? ooooh so confusing:^ Link to comment Share on other sites More sharing options...
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