can any one help me on bacterial rnase activity assay? which is the bst method for it?

What is the specificity of the enzyme from bacteria, as opposed to ribonuclease A, for example? Which assays have you considered so far?

Edited February 12, 201312 yr by BabcockHall

helo

i used kamekura and onishi method for rnase activity.briefly its steps r:

buffer pH 7

enzyme .1ml

rna 1mg

incubation for 2 hr

stop solution

centrifuge

then in end dilution of supernatent.

in many other protocol more or less all steps are same but i cant get the logic related to dilutions.

is it the necessary step or what??? as if i have the O.D 2.06 of test y i dilute it????

and if i dilute at which dilution i used???

ooooh so confusing:^