Vivalavida Posted November 10, 2012 Share Posted November 10, 2012 Hiii everyone, I really need some help... I would like to see if my γδ T-cells are activated or not, I've heard about the γδ T-cell isolation kit from Miltenyi BUT the problem is that they use an anti-γδ TCR antibody for the purification, and I'm afraid that this antibody will activate the cells while fixing thus make my results be wrong ... What am I supposed to do ?? Do I have to analyse by excluding all the other lymphocytes and then use an activating marker or what ,? Reallly need some help Tks Link to comment Share on other sites More sharing options...
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